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Nucleic Acids Research, 1983, Vol. 11, No. 24 8677-8690
© 1983

MOLECULAR BIOLOGY

Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes

S. Sharp1,*, T. Dingermann2, J. Schaack, J.A. Sharp1, D.J. Burke, E.M. DeRobertis+ and D. Söll

Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06511, USA +Biozentrum, University of Basel Klingelbergstrasse 70, CH-4056 Basel, Switzerland

*To whom correspondence should be addressed

Received August 4, 1983. Revised November 11, 1983. Accepted November 11, 1983.

The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophilla tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initiation site is a complex mechanism that involves the T-control region, the D-control region, and sequences 5' adjacent to the D-control region. In this study either "half" of the Drosophila tRNAArg gene promoted transcription in Xenopus oocytes. This finding supports a recent model for eukaryotic tRNA gene transcription (Dingermann et al., 1983, J. Biol. Chem. 258, 10395–10402) that proposes transcription initiation is dependent on ability of specific DNA sequences to sequester two RNA polymerase III transcription factors.

1Present address: Department of Microbiology and Molecular Genetics, California College of Medicine, University of California, Irvine, CA 92717, USA

2Present address: Institut fur Physiologische Chemie, Universitat Erlangen-Nurnberg, Fahrstrasse 17, D-8520 Erlangen, FRG


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