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Nucleic Acids Research, 1983, Vol. 11, No. 24 8931-8950
© 1983

MOLECULAR BIOLOGY

Isolation and characterization of two alleles of the chicken cytocbrome c gene

Keith J. Limbach and Ray Wu

Section of Biochemistry, Molecular and Cell Biology, Cornell University Ithaca, NY 14853, USA

Received August 9, 1983. Revised October 17, 1983. Accepted November 14, 1983.

Analysis of total chicken DNA by genomic blot hybridization indicates that only one cytochrome c gene exists in the chicken genome. The two alleles of this single cytochrome c gene have been isolated from a Charon 4A-chicken genomic library. This isolation made use of the yeast CYC1 cytochrome c gene as a specific hybridization probe. The 2 chicken alleles, CC9 and CC10, have been sequenced. The amino acid sequence predicted by these 2 alleles is identical, and agrees with the published chicken cytochrome c protein sequence. The flanking regions of these 2 alleles exhibit approximately 1% divergence, indicating a very limited polymorphism. Comparative sequence analysis with the flanking regions of previously isolated cytochrome c genes (yeast and rat) indicate no significant regions of homology. The presence of only one cytochrome c-like sequence in the chicken genome is in striking contrast with mammalian genomes, which contain as many as 20–30 cytochrome c-like sequences.


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