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Nucleic Acids Research, 1983, Vol. 11, No. 3 555-573
© 1983

MOLECULAR BIOLOGY

Structure and expression of cloned murine IFN-{alpha} genes

Gray D. Shaw*, Werner Boll, Hideharu Taira+, Ned Mantei, Peter Lengye§ and Charles weissmann

Institut für Molekularbiologie I, Universität ZOrich 8093 Zürich, Switzerland §§Yale University, Department of Molecular Biophysics and Biochemistry Box 6666 Yale Station, New Haven, CT 06511, USA

Received December 23, 1982. Accepted January 14, 1983.

The mouse has an interferon-a (MuIFN-a) gene family containing at least four, and likely more than ten members. A segment of mouse chromosomal DNA and cDNAs encoding murine alpha IFNs have been cloned, and the sequence of two MuIFN-a DNAs determined. No intron was found in the chromosomal gene. The two coding sequences produced biologically active IFN when expressed in monkey cells under the control of an SV40 promoter, and in E.coli under the control of the ampicillinase promoter. MuIFN-al had no detectable activity on human cells, while MuIFN-a2 was 201 as active on human as on mouse cells.

*Present address:Biogen Inc., 241 Binney Street, Cambridge, MA 02142, USA

+Department of Pure and Applied Sciences, University of Tokyo, 3-8-1 Komaba Meguro-ku Tokyo 153 Japan


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