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Nucleic Acids Research, 1983, Vol. 11, No. 3 591-604
© 1983

MOLECULAR BIOLOGY

Comparative structural analysis of cytoplasmic and chloroplastic 5S rRNA from spinach*

T. Pieler, M. Digweed, M. Bartsch1 and V. A. Erdmann

Institut für Biochemie, Freie Universität Berlin,Thielallee 69-73 1Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann Ihnestrasse 63-73, D-1000 Berlin 33, FRG

Received December 20, 1982. Accepted January 11, 1983.

5S rRNAs from Splnacea oleracea cytoplasmic and chloroplastic ribosomes have been subjected to digestion with the single strand specific nuclease S1 and to chemical modification of cytidines by sodium bisulphite in order to probe the RNA structure. According to these data, cytoplasmic 5S rRNA can be folded as proposed in the general eukaryotic 5S rRNA structure (1) and 5S rRNA from chloroplastides is shown to be more related to the general eubac-terial structure (2).

*On the occasion of his 60th birthday, we would like to dedicate this paper to professor Friedrich carner


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