Unmethlated domains in vertebrate DNA

doi:10.1093/nar/11.3.647

This Article

	Print PDF (3189K)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Add to My Personal Archive
	Download to citation manager
	Search for citing articles in: ISI Web of Science (102)
	Commercial Re-use Guidelines for Open Access NAR Content

Google Scholar

	Articles by Cooper, D. N.
	Articles by Bird, A. P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Cooper, D. N.
	Articles by Bird, A. P.

Social Bookmarking

	What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 3 647-658
© 1983

MOLECULAR BIOLOGY

Unmethlated domains in vertebrate DNA

David N. Cooper, Mary H. Taggart and Adrian P. Bird

MRC Mammalian Genome Unit, King's Buildings, West Mains Road Edinburgh EH9 3JT, UK

Received December 11, 1982. Accepted January 7, 1983.

We have detected a fraction that is rich in unmethylated HpaIIand Hhal sites by end-abelling HpaII fragments of chicken DNA.The fraction is not obvious when DNA fragments are stained withethidium bromide as it amounts to less than 2% of the genome.The average frequency of sites for HpaII is over thirteen timesgreater in the unmethylated fraction than in total DNA. Partialdigests indicate that the unmethylated sites are clustered inthe genome. Similar unmethylated fractions were detected insix other vertebrates in both somatic and germ line DNA.

Add to CiteULike CiteULike Add to Connotea Connotea Add to Del.icio.us Del.icio.us What's this?

This article has been cited by other articles:

C. Bock and T. Lengauer
Computational epigenetics
Bioinformatics, January 1, 2008; 24(1): 1 - 10.
[Abstract] [Full Text] [PDF]

B. Hendrich, J. Guy, B. Ramsahoye, V. A. Wilson, and A. Bird
Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development
Genes & Dev., March 15, 2001; 15(6): 710 - 723.
[Abstract] [Full Text]

N. Radomski, C. Kaufmann, and C. Dreyer
Nuclear Accumulation of S-Adenosylhomocysteine Hydrolase in Transcriptionally Active Cells during Development of Xenopus laevis
Mol. Biol. Cell, December 1, 1999; 10(12): 4283 - 4298.
[Abstract] [Full Text]

C. P. Walsh and T. H. Bestor
Cytosine methylation and mammalian development
Genes & Dev., January 1, 1999; 13(1): 26 - 34.
[Abstract] [Full Text]

H. A. McQueen, G. Siriaco, and A. P. Bird
Chicken Microchromosomes Are Hyperacetylated, Early Replicating, and Gene Rich
Genome Res., June 1, 1998; 8(6): 621 - 630.
[Abstract] [Full Text]

T. Watanabe, S. Inoue, H. Hiroi, A. Orimo, H. Kawashima, and M. Muramatsu
Isolation of Estrogen-Responsive Genes with a CpG Island Library
Mol. Cell. Biol., January 1, 1998; 18(1): 442 - 449.
[Abstract] [Full Text]

D Macleod, J Charlton, J Mullins, and A P Bird
Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island.
Genes & Dev., October 1, 1994; 8(19): 2282 - 2292.
[Abstract] [PDF]

G P Pfeifer, R L Tanguay, S D Steigerwald, and A D Riggs
In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1.
Genes & Dev., August 1, 1990; 4(8): 1277 - 1287.
[Abstract] [PDF]

M Holler, G Westin, J Jiricny, and W Schaffner
Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated.
Genes & Dev., September 1, 1988; 2(9): 1127 - 1135.
[Abstract] [PDF]

				B. Hendrich, J. Guy, B. Ramsahoye, V. A. Wilson, and A. Bird Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development Genes & Dev., March 15, 2001; 15(6): 710 - 723. [Abstract] [Full Text]

				N. Radomski, C. Kaufmann, and C. Dreyer Nuclear Accumulation of S-Adenosylhomocysteine Hydrolase in Transcriptionally Active Cells during Development of Xenopus laevis Mol. Biol. Cell, December 1, 1999; 10(12): 4283 - 4298. [Abstract] [Full Text]

				C. P. Walsh and T. H. Bestor Cytosine methylation and mammalian development Genes & Dev., January 1, 1999; 13(1): 26 - 34. [Abstract] [Full Text]

				H. A. McQueen, G. Siriaco, and A. P. Bird Chicken Microchromosomes Are Hyperacetylated, Early Replicating, and Gene Rich Genome Res., June 1, 1998; 8(6): 621 - 630. [Abstract] [Full Text]

				T. Watanabe, S. Inoue, H. Hiroi, A. Orimo, H. Kawashima, and M. Muramatsu Isolation of Estrogen-Responsive Genes with a CpG Island Library Mol. Cell. Biol., January 1, 1998; 18(1): 442 - 449. [Abstract] [Full Text]

				D Macleod, J Charlton, J Mullins, and A P Bird Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island. Genes & Dev., October 1, 1994; 8(19): 2282 - 2292. [Abstract] [PDF]

				G P Pfeifer, R L Tanguay, S D Steigerwald, and A D Riggs In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1. Genes & Dev., August 1, 1990; 4(8): 1277 - 1287. [Abstract] [PDF]

				M Holler, G Westin, J Jiricny, and W Schaffner Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated. Genes & Dev., September 1, 1988; 2(9): 1127 - 1135. [Abstract] [PDF]