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Nucleic Acids Research, 1983, Vol. 11, No. 3 883-891
© 1983

MOLECULAR BIOLOGY

Sequence of the nucleocapsid gene from murine coronavirus MHV-A59

John Armstrong+, Sjef Smeekens and Peter Rottier

Institute of Virology, Veterinary Faculty State University of Utrecht 3508 TD Utrecht, The Netherlands +European Molecular Biology Laboratory Postfach 10.2209, D-6900 Heidelberg, FRG

Received December 1, 1982. Accepted January 5, 1983.

The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has heen determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector Ml 3 mp8 for direct sequence determination. A sequence of 1817 nu-cleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues.


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