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Nucleic Acids Research, 1983, Vol. 11, No. 4 1077-1097
© 1983

MOLECULAR BIOLOGY

Yeast killer dsRNA plasmids are transcribed in vivo to produce full and partial-length plus-stranded RNAs

Keith A. Bostian*, Virginia E. Burn*, S. Jayachandran+ and Donald J. Tipper+

*Division of Biology and Medicine, Brown University Providence, RI 0291 +Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School Worcester, MA 01605, USA

Received November 16, 1982. Revised January 18, 1983. Accepted January 18, 1983.

In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l, m) and partial-length (la, ma) single–stranded species. Both L–dsRNA transcripts (l, la) havein vitro mRNA activity for L-Pl, previously shown to be identical to ScV-Pl, the 88,000 dalton major capsid protein of the virus-like particles containing L- and M –dsRNAs. 1, but not la, is bound to poly (U) -sepharose and may be polyadenylated. Other L-dsRNA gene products and their transcripts may exist. For M-dsRNA, both species (m, ma) have in vitro mRNA activity for M,–P1, the 32,000 dalton pre-protoxin encoded by M1 -dsRNA. Both m and ma are bound to poly(U) –Sepharose and ma is probably a 5' terminal fragment of m. A functional model for M1 -dsRNA killer plasmid structure is presented.


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