Nucleic Acids Research, 1983, Vol. 11, No. 4 1123-1131
© 1983
MOLECULAR BIOLOGY |
The nucteotide sequence of myodn light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue
1University of Hamburg, Department of Toxicology. Medical School Hamburg Hamburg, FRG 2Roche Institute of Molecular Biology Nutley, NJ 07110, USA
*To whom requests for reprints should be addressed
Received October 15, 1982. Revised December 15, 1982. Accepted January 10, 1983.
The nucleotide sequence of a cDNA clone (pML10) for chicken cardiac myosin light chain is described. The cDNA insert contains 613 nucleotides representing the entire coding sequence, with the exception of nine NH2tenrnnal ammo acids, and the full 3'non-coding region of 146 nucleotides. The missing 5' terminus of the mRNA, not represented in the clone pML10, was obtained by extension of the cDNA using a 43 nucleotide long internal EcoRI fragment as a primer. The non-coding region contains several direct and inverted repeated sequences and the polyadenylation signal sequence AATAAA. The coding portion exhibits non-random usage of synonymous codons with a strong bias for codons ending in G and C.