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Nucleic Acids Research, 1983, Vol. 11, No. 5 1213-1226
© 1983

MOLECULAR BIOLOGY

Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA

Judith Chebath*, Gilles Merlin, Richard Metz, Phillippe Benech and Michel Revel

Department of Virology, The Weizmann Insitute of Science Rehovot, Israel

Received January 31, 1983. Accepted February 15, 1983.

Treatment of responsive cells by interferons (IFNs) induces within a few hours a rise in the concentration of several proteins and mRNAs. In order to characterize these IFN-induced mRNA species, we have cloned in E. coli the cDNA made from a 17–18S poly(A)+ RNA of human fibroblastoid cells (SV80) treated with IFN-ß. We describe here a pBR322 recombinant plasmid (C56) which contains a 400 bp cDNA insert corresponding to a 18S mRNA species newly induced by IFN. The C56 mRNA codes for a 56,000 dalton protein easily detectable by hybridization-translation experiments. The sequence of 66 of the carboxy-terminal amino-acids of the protein can be deduced from the cDNA sequence. IFNs-{alpha}, ß or {gamma} are able to activate the expression of this gene in human fibroblasts as well as lymphoblastoid cells. The mRNA is not detectable without IFN; it reaches maximum levels (0.1% of the total poly(A)+ RNA) within 4–8 hrs and decreases after 16 hrs.

*Present address: Unite 38 INSERM 27 Bd J. Moulin, 13385, Marseille, Cedex 4, France.


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