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Nucleic Acids Research, 1983, Vol. 11, No. 5 1267-1281
© 1983

MOLECULAR BIOLOGY

Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins

Alan J. Cann, Glyn Stanway, Rudi Hauptmann*, Philip D. Minor+, Geoffrey C. Schild+, Lindsay D. Clarke, Roger C. Mountford and Jeffrey W. Almond

Department of Microbiology, Leicester University Medical School University Road, Leicester LEI 7RH +National Institute for Biological Standards and Control Holly Hill, London NW3 6RB UK

Received January 14, 1983. Revised February 16, 1983. Accepted February 16, 1983.

Overlapping cDNA clones representing the entire genone of poliovirus type 3 have been prepared in E. coli by two separate methods. Cloning of RNA.cDNA hybrids produced a more comprehensive set of clones with generally larger cDNA inserts than cloning of double - stranded cDNA. A restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3' terminus, comprising the region encoding the protease and polymerase proteins, are presented.

*Present address: Ernst-Boehringer-Institute for Arzneimittelforschung, Bender & Co. mbH, Dr Boehringer-Gasse 5–11, A-1121 Vienna, Austria


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