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Nucleic Acids Research, 1983, Vol. 11, No. 5 1375-1388
© 1983


MOLECULAR BIOLOGY

Cross-reaction of snRNA and an Alu I-like sequence from rat with DNAs from different eucaryotic species

Nikolaus Blin, Thomas Weber and Angel Alonso*

German Cancer Research Center, Institute of Experimental Pathology Im Neuenheimer Feld 280, 6900 Heidelberg, FRG

*To whom correspondence should be addressed.

Received December 13, 1982. Revised February 7, 1983. Accepted February 7, 1983.

Sequence homologies to rat Ul-snRNA and U2-snRNA were investigated in DNA from 23 eucaryotic species (3 lower eucaryotes, 4 plants, and 16 animals) using dot hybridization at various stringency conditions. Cross-hybridization among very distantly related species in e.g. plants-insects or mold-vertebrates is not the rule; there are, however, examples for stronger ho-mologies like Rattus-Dictyostelium.

Furthermore, DNA from all 23 species was analysed for sequence homologies with the repetitive DNA sequence Bl (an Alu I family equivalent) from rat. We observed a wide range of homologies covering some plants and insects, up to vertebrates. Hybridization at increasing stringency conditions revealed species with higher degree of homology to the rat Bl sequence: maize, chicken, and hamster.


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