Nucleic Acids Research, 1983, Vol. 11, No. 5 1475-1489
© 1983
MOLECULAR BIOLOGY |
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
Departments of Biological Chemistry and Genetics, Division of Biology and Biomedical Sciences Washington University School of Medicine, St. Louis, MO 63110, USA
*To whom correspondence should be addressed.
Received November 8, 1982. Revised January 18, 1983. Accepted January 18, 1983.
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
+Permanent address: Department of Biochemistry, University of Mississippi Medical Center, Jackson, MS 39216, USA
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