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Nucleic Acids Research, 1983, Vol. 11, No. 5 1543-1553
© 1983

MOLECULAR BIOLOGY

Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis*

Walter Knöchel, Wolfgang Meyerhof, Sabine Hummel and Ulrich Grundmann

Institut f{euro}r Molekularbiologie und Biochemie, Freie Universität Berlin Arnimallee 22, D-1000 Berlin 33, FRG

Received January 11, 1983. Revised February 10, 1983. Accepted February 14, 1983.

Globin mRNA was isolated from immature red blood cells of an adult Xenopus laevis female. mRNA/cDNA hybrids were integrated in the Pst I cleavage site of pBR 322 by G/C tailing, and cloned in Escherichia coli strain HB 101. By restriction site analysis as well as hybridization behaviour we identified two clones coding for minor adult {alpha} and ß globin chains. Nucleotide sequence analysis and derived amino acid sequences are presented.

*This article is dedicated to Prof. Dr. Heinz Tiedemann on the occasion of his 60th birthday


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