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Nucleic Acids Research, 1983, Vol. 11, No. 5 1555-1566
© 1983

MOLECULAR BIOLOGY

The sequence of RNA segment 1 of influenza virus A/NT/60/68 and its comparison with the corresponding segment of strains A/PR/8/34 and A/WSN/33

K.L. Jones, J.A. Huddleston and G.G. Brownlee

Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford OX1 3RE, UK

Received January 7, 1983. Revised February 2, 1983. Accepted February 2, 1983.

The complete nucleotide sequence of RNA segment 1 of influenza virus A/NT/60/68, corresponding to the PB2 protein, has been determined. It is 2341 nucleotides long, encoding a predicted product of 759 amino acids with a net charge of +271/2 at neutral pH. The predicted amino acid sequence has been compared to the equivalent sequences in influenza viruses A/PR/8/34 and A/WSN/33. Evolutionary divergence, assuming a direct lineage from A/PR/8/34 and allowing for "laboratory drift", is 0.08% per year. The alignment of RNA segment 10 of A/NT/60/68 with segments 1 and 3 is completed, confirming that it is a mosaic of regions from these two segments.


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