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Nucleic Acids Research, 1983, Vol. 11, No. 5 1579-1588
© 1983

CHEMISTRY

Raman diagnosis of nucleic acid structure: sugar-puckering and glycosidic conformation in the guanosine moiety

Yoshifumi Nishimura, Faculty of Pharamaceutical Sciences*, Masamichi Tsuboi, Faculty of Pharamaceutical Sciences*, Toru Nakano+, Shigesada Higuchi+, Tomohiro Sato§, Toshio Shida**, Seiichi Uesugi**, Eiko Ohtsuka** and Morio Ikehara

*University of Tokyo Hongo, Bunkyo-ku +Mitsubishi-Kasei Institute of Life Science 11 Minamiooya, Machida, Tokyo, Japan

Received December 13, 1982. Revised February 15, 1983. Accepted February 15, 1983.

Observations of Raman spectra of various nucleic acids indicate that the guanine ring breathing frequency is sensitive to the internal rotation angle around the glycosidic bond and to the conformation of the five-membered ring of the ribose residue that is directly connected with the guanine residue in question. It is found that 682 cm–1 for C2'-endo-anti, at 665 cm–1 for C3–-endo-anti, and at 625 cm–1 for C3'-endo-syn. A DNA octamer d(pGpApApTpTpCpC) shows, in its aqueous solution, a broad Raman band at 680 cm–1 with a tail at 670 cm–1. This fact suggests that the guanosine residues in this oligomer take primarily C2'-endo-anti conformation but an appreciable amount of fluctuation of the ribose ring structure towards C3'-endo is involved.

§Shionogi Research Laboratory, Shionogi & Co., Ltd., Fukushima-ku, Osaka, Japan

**Faculty of Pharamaceutical Sciences, Osaka University, Suita, Osaka, Japan.


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