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Nucleic Acids Research, 1983, Vol. 11, No. 6 1873-1880
© 1983

MOLECULAR BIOLOGY

Spacing mutations between the Escherichia coli PBAD RNA polymerase binding site and the araC (I) induction site

William Hendrickson, Joanne Petithory and Robert Schleif

Biochemistry Department, Brandeis University Waltham, MA 02254, USA.

Received November 18, 1982. Accepted February 11, 1983.

Mutations in the Escherichia coli promoter PBAD have been constructed which alter the spacing of the adjacent RNA polymerase and araC inducer protein binding sites. While deletion of a single base-pair or small insertions do not detectably affect araC protein binding to DNA and they do not alter the conserved sequence of the RNA polymerase binding site, stimulation of PBAD in vivo is greatly reduced. The experiments suggest that the distance or angle between the two proteins on the DNA is critical for promoter function.


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