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Nucleic Acids Research, 1983, Vol. 11, No. 7 1957-1968
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence of a ‘truncated rRNA operon’ of the Euglena gracilis Chloroplast genome

Entienne Roux, Lucia Graf+ and Erhard Stutz*

Laboratoire de Biochimie, Université de Neuchâtel, Ch. de Chantemerle 18, CH-2000 Neuchâtel Switzerland

*To whom correspondence should be addressed

Received February 18, 1983. Revised March 11, 1983. Accepted March 11, 1983.

An extra 16S rRNA gene (s-16S rDNA) from the Euglena gracilis chloroplast genome and several hundred positions of its flanking regions have been sequenced. The structural part has 1486 positions and is to 98% homologous in its sequence with the 16S rRNA gene in functional chloroplast rRNA operons. Sequences of about 200 positions upstream and 15 positions downstream of the structural part of the s-16S rRNA gene region are highly homologous with corresponding parts in the functional operon. Neither tRNA genes (Ala, Ile) nor parts of the 23S and 5S rRNA genes are found within 557 positions after the 3' end of the S-16S rRNA gene, i.e., the 330 bp homology, observed in electron microscopic studies of heteroduplexes (4), between the S-16S rDNA downstream region and the 6.2 kb repeated segment containing the functional rRNA operon, must be due to a DNA stretch in the interoperon spacer. A structural model of the "truncated rRNA operon" is presented. Results from S-l endonuclease analysis suggest that the S-16S rDNA region is probably not transcribed into stable S-16S rRNA.

+Present address: Friedrich Miescher-Institute, P.O. Box 2543, CH-4002 Basel, Switzerland


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