Skip Navigation

This Article
Right arrow Print PDF (2539K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lyamichev, V. I.
Right arrow Articles by Lyubchenko, Yu. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lyamichev, V. I.
Right arrow Articles by Lyubchenko, Yu. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 7 2165-2176
© 1983

MOLECULAR BIOLOGY

Localization of low-melting regions in phage T7 DNA

V. I. Lyamichev, I. G. Panyutin, D. I. Cherny and Yu. L. Lyubchenko

Institute of Molecular Gentics, USSR Academy of Sciences Moscow, USSR

Received February 8, 1983. Accepted March 8, 1983.

Specific fragmentation of T7 DNA at glyoxal–fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5–1.2;14.8±0.3;46.3±0.5; 98.4±0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
L. Shokri, M. J. McCauley, I. Rouzina, and M. C. Williams
DNA Overstretching in the Presence of Glyoxal: Structural Evidence of Force-Induced DNA Melting
Biophys. J., August 1, 2008; 95(3): 1248 - 1255.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.