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Nucleic Acids Research, 1983, Vol. 11, No. 8 2273-2285
© 1983

MOLECULAR BIOLOGY

The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end

Gert-Jan B.van Ommen*, Annika C. Arnberg§, Frank Baas, Huguette Brocas+, Andrè Sterk, Wil H.H. Tegelaers, Gilbert Vassart+ and Jan J.M.de Vijlder

Department of Pediatric Endocrinology, Academical Medical Center GV2-131, Meibergdreef 9, 1105 AZ Amsterdam §Biochemical Laboratory, The University Groningen, The Netherlands +IRIBHN, Campus Hôpital Erasme Route de Lennik 808, B-1070 Brussels, Belgium

*To whom correspondence should be sent

Received March 1, 1983. Accepted March 25, 1983.

We have cloned overlapping segments of the human thyroglobulin gene from a genomic cosmid library. Restriction mapping and electron microscopy show that a region of 38 kb at or near the 3'-end of this gene encodes only 850 nucleotides or 10% of the messenger RNA (mRNA) sequence. The region contains five exons of 130–210 nucleotides, split by introns of 1 to 15–17 kb. This represents the lowest ratio of coding to non-coding DNA (2.2%) found thusfar in any eukaryotic gene. Blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences


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