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Nucleic Acids Research, 1983, Vol. 11, No. 8 2325-2335
© 1983

MOLECULAR BIOLOGY

Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX

Michael Jaye, Henri de la Salle, Fabienne Schamber, Alain Balland, Vipin Kohli, Annie Findeli, Paul Tolstoshev and Jean-Pierre Lecocq

Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

Received February 22, 1983. Accepted March 22, 1983.

A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.


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