Nucleic Acids Research, 1983, Vol. 11, No. 8 2447-2464
© 1983
MOLECULAR BIOLOGY |
The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter
Laboratoire de Gènètique Molèculaire des Eucaryotes du CNRS, Unitè 184 de Biologie Molèculaire et de Gènie Gènètique de 1'INSERM, Institut de Chimie Biologique–Facultè de Mèdecine 11, Rue Humann, Strasbourg 67085 Cedex, France
Received March 1, 1983. Revised March 24, 1983. Accepted March 24, 1983.
We have constructed deletion and point mutations within the Simian virus 40 (SV40) early promoter region which contains two tandemly repeated 21 bp sequences and a related 22 bp sequence (the "upstream" 21 bp repeat region). After transfection into permissive CV-1 cells and non-permissive mouse 3T3-4E cells, the effect of the mutations on early gene expression was studied by measuring T-antigen production, using indirect immunofluoresence. Our results demonstrate that the 21 bp repeat region, and in particular the six GC-rich motifs 5'-CCGCCC-3' which are repeated in this region constitute an important element of the SV40 early promoter. Surprisingly, we found that the requirement for the 21 bp repeat region for early gene expression was partially fulfilled even when it was in the inverted orientation.
*Present address: Medical Research Council, Virology Unit, Church Street, Glasgow, G11 5JR, UK.
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