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Nucleic Acids Research, 1983, Vol. 11, No. 9 2563-2573
© 1983

MOLECULAR BIOLOGY

Isolation of the messenger RNA for 8S DNA ligase in early developing axolotl egg and its cell free translation

Pierre Thiebaud1, J. Lefresne2, J. Signoret2 and Jean-Claude David1,*

1Laboratoire de Biochimie du Devdoppement C.N.R.S. LA No 256, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex 2Laboratoire de Biologie du Développement, Faculté des Sciences 14000 Caen, France

*To whom correspondence should be addressed

Received March 1, 1983. Accepted April 6, 1983.

A new DNA ligase activity is expressed when the Axolotl eggs enter cleavage. The messenger RNA can be labelled by [3H] uridine thereby indicating its de novo synthesis. This new genetic expression is occuring just before cleavage and is the earliest found during Amphibian development. The newly synthesized [3H] mRNA can be translated in vitro in the rabbit reticulocyte lysate system. The resulting product is a 160 K protein specifically iramuno-precipitated with the antiserum directed against 8S DNA ligase. This in vitro translated polypeptide exhibits 8S DNA ligase activity specific of activated or fertilized eggs but does not display 6S DNA ligase activity of non activated eggs.


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