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Nucleic Acids Research, 1983, Vol. 11, No. 9 2617-2626
© 1983

MOLECULAR BIOLOGY

The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region

Satoshi Tabata*, Atsuhiro Oka*, Kazunori Sugimoto*, Mituru Takanami*,+, Soichi Yasuda§ and Yukinon Hirota§

*Institute for Chemical Research, Kyoto University Uji, Kyoto 611 §National Institute of Genetics Mishima, Shizuoka 411, Japan

+To whom correspondence should be addressed

Received February 17, 1983. Revised March 28, 1983. Accepted March 28, 1983.

The replication origin of the E.coli K-12 chromosome has been isolated as autonomously replicating molecules (oriC plasmid), and the DNA region essential for replicating function (oriC) has been localized to a sequence of 232–245 base-pairs(bp) by deletion analysis. In this report, the functional role of oriC was analysed by using an in vitro replication system and various OriC* and OniC– plasmids previously constructed. The results obtained were summarized as follows: (1) The oriC sequence contained information enough to direct bidirectional replication. (2) The actual DNA replication began at a region near, but outside, oriC and progressed bidirectionally. (3) Initiation of DNA synthesis at the specific region required the dnaA-complementing fraction from cells harboring a dnaA-carrying plasmid.


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