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Nucleic Acids Research, 1983, Vol. 11, No. 9 2665-2679
© 1983

CHEMISTRY

Quantitation of cation binding to wheat germ ribosomes: Influences on submit association equilibria and ribosome activity

Joan M. Sperrazza1 and Linda L. Spremulli2,*

Departments of Biochemistry and Nutrition, The University of North Carolina Chapel Hill, NC 27514, USA *Departments of Biochemistry and Nutrition, Chemistry, The University of North Carolina Chapel Hill, NC 27514, USA

2To whom correspondence should be addressed.

Received February 8, 1983. Revised April 5, 1983. Accepted April 5, 1983.

The binding of Mg2+, spermine, and spermidine to wheat germ ribosomes was quantitated following equilibrium dialysis. The Mg2+ binding data demonstrate that Mg2+ and K+ compete for binding to the ribosomes. Mg2+ binding saturates at approximately 0.56 positive charges per phosphate (+/P). The Mg , spermine and spexmidine binding data indicate that either polyamine replaces Mg2+ upon binding to the ribosomes. Mg2+ and polyamine binding combined saturates at approximately 0.29 +/P under the conditions reported. When a critical number of Mg2+ ions are replaced by either polyamine, the activity of the ribosomes falls dramatically. Ribosomal subunit association increases with the degree+of phosphate charge neutralization due to the binding of Mg2+ Total charge neutralization during subunit association by Mg and polvamine binding combined, is much less than that achieved by Mg2+ alone.

1Present Address: Cancer Research Center; University of North Carolina, Chapel Hill, NC 27514.


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