Skip Navigation

This Article
Right arrow Print PDF (4718K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Reeves, R.
Right arrow Articles by Howard, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reeves, R.
Right arrow Articles by Howard, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 9 2681-2700
© 1983

MOLECULAR BIOLOGY

In vivo incorporation of Drosophila H2a histone into mammalian chromatin

Raymond Reeves*, Cornelia M. Gorman+ and Bruce Howard+

*Biochemistry/Biophysics Program, Washington State University Pullman, WA 99164 +Laboratory of Molecular Biology, National Cancer Institute Bethesda, MD 20205, USA

Received January 20, 1983. Revised April 6, 1983. Accepted April 6, 1983.

Hybrid prokaryot1c/eukaryotic expression vectors have been used to introduce Drosophila histone genes into CV-1 African green monkey tissue culture cel Is. Transfection of CV-1 cells with Drosophila genes under the control of Insect DNA promoter sequences results 1n low level expression of histone genes. On the other hand, when the Drosophila H2a gene 1s juxtaposec downstream from the long terminal repeat sequence of Rous sarcoma virus (RSV) expression of the Insect gene is considerably more efficient; both 3' polyadenylated insect histone messenger RNA and putative Drosophila H2a histone protein can be readily detected 1n the transduced cells. Using this RSV/H2a vector, we have been able to demonstrate the presence of Drosophila H2a histone 1n monaner nucleosome preparations isolated from transfected CV-1 cells. These results suggest the feasibility of ‘remodeling’ cellular chromatin 1n vivo 1n precisely defined ways. The techniques described may be generally applicable to other genes coding for chromosomal proteins.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.