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Nucleic Acids Research, 1983, Vol. 11, No. 9 2717-2731
© 1983

MOLECULAR BIOLOGY

Stability of the conservative mode of nucleosome assembly

I.M. Leffak

Department of Biochemistry, Wright State University, School of Medicine Dayton, OH 45435, USA

Received January 14, 1983. Revised March 20, 1983. Accepted March 20, 1983.

The conservative assembly of nucleosome histone octamer cores has been confirmed by electrophoretic analysis of density labeled histones following equilibrium buoyant density centrifugation. After normal replication, crosslinked octamers are shown not to contain a mixture of new and old core hiatonee. Moreover, when DNA synthesis is inhibited by ara-C nucleoaome cores are still assembled exclusively from nascent histone. Similarly, after release from cycloheximide inhibition newly synthesized core histone is conservatively deposited. Thus, a conservative mechanism of histone octamer assembly occurs when nascent histone is present in the normal stoichiometry to nascent DNA and when chromatin is assembled in nascent histone or nascent DNA excess.


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