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Nucleic Acids Research, 1983, Vol. 11, No. 9 2881-2891
© 1983

MOLECULAR BIOLOGY

Complete nucleotide sequence of alfalfa mosaic virus RNA3

R.F. Barker, N.P. Jarvis, D.V. Thompson, L.S. Loesch-Fries and T.C. Hall

Agrigenetics Advanced Research Laboratory 5649 East Buckeye Road, Madison, WI 53716, USA

Received February 14, 1983. Revised March 31, 1983. Accepted March 31, 1983.

A full-length cDNA clone of alfalfa mosaic virus (AMV) RNA3 was prepared and sequenced. The 2,037 base sequence contains two open reading frames of 903 and 666 nucleotldes that code for a 32,400 dalton protein (32.4K protein) and the 24,380 dalton coat protein, respectively. A 5'-noncodlng sequence of 240 bases preceedlng the 32.4K protein contains homologous regions that may have a function in its translation. The interclstronic junction is 49 bases long, the last 36 bases representing the 5'-end of the subgenomic RNA4. The remaining 179 bases comprise the 3'-termlnal noncoding sequence.


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