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Nucleic Acids Research, 1984, Vol. 12, No. 10 4097-4109
© 1984

MOLECULAR BIOLOGY

A DNA fragment from the human X chromosome short arm which detects a partially homologous sequence on the Y chromosomes long arm

Michel Koenig, Giovanna Camerino1, Roland Heilig and Jean-Louis Mandel

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génénétique de I'INSERM, Faculté de Médecine 11, Rue Humann, 67085 Strasbourg Cedex, France

Received March 29, 1984. Accepted May 1, 1984.

An X linked human DNA fragment (named DXS31) which detects partially homologous sequences on the Y chromosome has been isolated. Regional localisation of the two sex linked sequences was determined using a panel of rodent-human somatic cell hybrids. The X specific sequence is located at the tip of the short arm (Xp22.3-pter), i.e. within or close to the region which pairs with the Y chromosome short arm at meiosis. However the Y specific sequence is located in the heterochromatic region of the long arm (Yq11-qter) and lies outside from the pairing region. DNAs from several XX male subjects were probed with DXS31 and in all cases a double dose of the X linked fragment was found, and the Y specific fragment was absent. DXS31 detects in chimpanzee a male-female differential pattern identical to that found in man. However results obtained in a more distantly related species, the brown lemur, suggest that the sequences detected by DXS31 in this species might be autosomally coded. The features observed with these X-Y related sequences do not fit with that expected from current hypothesises of homology between the pairing regions of the two sex chromosomes, nor with the pattern observed with other X-Y homologous sequences recently characterized. Our results suggest also that the rule of conservation of X linkage in mammals might not apply to sequences present on the tip of the X chromosome short arm, in bearing with the controversial issue of steroid sulfatase localisation in mouse.

1Permanent address: Dipartimento di Gerietica e Microbiologia, Universita di Pavia, via S. epifanio, 27100 Pavia, Italy


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