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Nucleic Acids Research, 1984, Vol. 12, No. 10 4247-4258
© 1984

CHEMISTRY

Interaction of Z form poly(dG-dC). poly(dG-dC) with divalent metal ions: localization of the binding sites by I.R. spectroscopy

J.A. Taboury, P. Bourtayre, J. Liquier and E. Taillandier*

Laboratoire de Spectroscopie Biomoléculaire, UER de Médecine, Université Paris XIII 74, rue Marcel Cachin, 93000 Bobigny, France

*To whom correspondence should be addressed

Received April 5, 1984. Accepted May 1, 1984.

The secondary structures of poly(dG-dC).poly(dG-dC) in the presence of alcaline, alcaline earth and first row transition metal ions (Na+, Mg2+ Co2+ Ni2+) are investigated by infrared spectroscopy. The conformational transitions are studied as a function of the hydration of the polynucleotide and counterion nature and content. The use of selectively deuterated poly(dG-dC).poly(dG-dC) on the 8-carbon of guanines allows to show that a direct interaction occurs between the N7 site of guanines and the transition metal ions Co2+ and Ni2+. In the case of Mg2+, for high ion/nucleotide ratios, the interaction occurs essentially at the level of the phosphate groups. This interaction leads to a modification of the left-handed conformation. Based on the IR spectroscopy results, an explanation is proposed for the different efficiencies of these various ions to induce the B -> Z transition.


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