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Nucleic Acids Research, 1984, Vol. 12, No. 10 4351-4359
© 1984

CHEMISTRY

Effects of pH on the stability of chromatin core particles

Louis J. Libertini and Enoch W. Small

Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331, USA

Received February 6, 1984. Revised April 25, 1984. Accepted April 25, 1984.

Chromatin core particles near physiological ionic strength undergo a reversible transition induced by changes in pH near neutrality. While sedimentation studies indicate no significant effect on size or shape, changes in tyrosine fluorescence anisotropy and in circular dichroism suggest a somewhat looser structure at high pH. Further support of this suggestion is given by high salt dissociation experiments; at pH 8 core particles begin to show changes at lower salt concentration than at pH 6. The pH transition appears unaffected by the presence of Mg2+ but can be blocked by crosslinking of the histones. A possible relationship is suggested between this transition and increases in intracellular pH which correlate with enhancement in several aspects of cellular activity including DNA replication.


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