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Nucleic Acids Research, 1984, Vol. 12, No. 11 4445-4453
© 1984

CHEMISTRY

Chemical conversion of cytidine residues into 4-thiouridines in yeast tRNAPhe. Determination of the modified cytidines

Nadine Riehl, Philippe Carbon, Bernard Ehresmann and Jean-Pierre Ebel

Institut de Biologie Moléculaire et Cellulaire du CNRS 15 rue René Descartes, 67084 Strasbourg, France

Received March 21, 1984. Revised May 8, 1984. Accepted May 8, 1984.

Treatment of yeast phenylalanine tRNA with pressurized hydrogen sulfide results in conversion of cytidine residues into 4-thiouridine residues. Under conditions leading to an average modification of one cytidine per tRNA molecule 9 positions are thiolated. The 4-thiouridine residues are distributed along the tRNA molecule. Four of the reactive cytidines are located in single-stranded regions: Cm32, C60, C74 and C75. The five others are located in base pairs: C2, C27, C56, C61 and C63. Importance of replacement of an ami no group by a thiol group on hydrogen bonding and on biological activity of the modified tRNA is discussed.


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