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Nucleic Acids Research, 1984, Vol. 12, No. 11 4507-4516
© 1984

ENZYMOLOGY

A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus

Bo-Qin Qiang* and Ira Schildkraut

New England Biolabs, Inc. 32 Tozer Road, Beverly, MA 01915, China *Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Beijing, China

Received February 28, 1984. Revised May 8, 1984. Accepted May 8, 1984.

A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus. This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN/NGGCC, symmetrically to produce a three base, 3' extension.


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