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Nucleic Acids Research, 1984, Vol. 12, No. 11 4517-4529
© 1984

MOLECULAR BIOLOGY

Analysis of the mouse {gamma}-crystallin gene family: assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene

Si Lok1, Lap-Chee Tsui1,2, Toshimichi Shinohara3, Joram Piatigorsky3, Reynold Gold1 and Martin Breitman1,2,*

1Dept. Medical Genetics, Univ. Toronto Toronto, Ontario M5S 1A8, Canada 2Dept. Genetics, The Hospital for Sick Children, 555 University Avenue Toronto, Ontario M5G 1X8, Canada 3Laboratory of Molecular & Developmental Biology, National Eye Institute Bethesda, MD 20205, USA

* Address correspondence to: Dr M.L.Breitman, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada

Received February 23, 1984. Revised May 7, 1984. Accepted May 7, 1984.

Blot hybridization analysis of mouse DNA with {gamma}-crystallin-specific CDNAs has detected the presence of a multigene family comprised of at least four related genes. The detailed structure of one of these genes, mouse {gamma}4-crystallin (M{gamma}4.1), and its corresponding cDNA has been determined. The gene spans approximately 2.6 kilobases (kb) and contains two introns. The gene predicts a polypeptide of 174 amino acids that shares extensive sequence homology with {gamma}-crystallin polypeptides of other species. The two similar structural domains of the protein correspond exactly to the second and third exons of the gene, supporting an exon-duplication model of gene evolution. The similarity in structure of this gene to that recently reported for a {gamma}-crystallin gene of the rat (1) suggests that a common structure may exist for all {gamma}-crystallin genes of the two species. Moreover, a highly conserved region, 50 nucleotides in length, immediately precedes the TATA box of both the mouse and rat genes, suggesting that this sequence may be important in gene regulation.


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