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Nucleic Acids Research, 1984, Vol. 12, No. 11 4625-4635
© 1984

CHEMISTRY

31P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3, and (dC-dG)4 in high salt solution

Tadeusz A. Holak, Philip N. Borer, George C. Levy*, Jacques H. van Boom1 and Andrew H.-J. Wang2

NIH Research Resource for Multi-Nuclei NMR & Data Processing, Dept. Chemistry, Syracuse University Syracuse NY 13210, USA 1Dept. Organic Chemistry, University of Leiden The Netherlands 2Dept. Biology, Massachusetts Institute of Technology Cambridge, MA 02139, USA

*To whom correspondence should be addressed

Received March 26, 1984. Accepted May 9, 1984.

In 4H NaCl solutions (dC-dG)n (n=3,4; {small tilde}9mM) exist as a mixture ot B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). {Delta}H=–44 and –17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0–57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.


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