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Nucleic Acids Research, 1984, Vol. 12, No. 11 4665-4677
© 1984

MOLECULAR BIOLOGY

Nocleotide sequence of tbe tmr locus of Agrobacterium tumefaciens pTi T37 T-DNA

S.B. Goldberg, J.S. Flick and S.G. Rogers*

Monsanto Company, 800 North Lindbergh Boulevard Saint Louis, MO 63167, USA

*To whom correspondence should be addressed

Received March 5, 1984. Revised May 16, 1984. Accepted May 16, 1984.

The nucleotide sequence of the tmr locus from the nopaline-type pTi T37 plasmid of Agrobacterium tumefaciens was determined. Examination of this sequence allowed us to identify an open reading frame of 720 nucleotides capable of encoding a protein with a derived molecular weight of 27025 d. Comparison of the pTi T37 tmr sequence with the published sequence of the pTi Ach5 tmr locus shows over 88% homology in the 240 bases 5' to the translational initiation codon and over 91% homology in the coding sequences. The 3' nontranslated regions show less than 50% homology as expected for the 3' regions of divergent related genes. The possible significance of areas of conserved sequences, particularly in the 5' regulatory regions, is discussed.


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