Nucleic Acids Research, 1984, Vol. 12, No. 12 4849-4863
© 1984
MOLECULAR BIOLOGY |
Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant
Department of Microbiology and Molecular Genetics, University of California Irvine, CA 92717, USA
Received April 23, 1984. Accepted May 21, 1984.
The Tn10 tetR gene encodes the repressor that regulates transcription of the Tnl0 tetracycline resistance determinant. We have determined the DNA sequence of the tetR gene and a 905 base pair region immediately 3' to tetR. The tetR gene is located on a 701 base pair HincII restriction fragment. Deletions at either end of this region eliminate synthesis of the wild-type tetR protein in E. coli minicells, and eliminate tetR activity as measured by repression of ß-galactosidase synthesis in tetA-lacZ operon fusion strains. Taken together, the DNA sequence and the genetic data indicate that tetR encodes a 207 amino acid protein with a calculated molecular weight of 23,328. This value is in good agreement with estimates of 23,000–25,000 based on electrophoretic mobility in SDS-polyaerylamide gels. There is 47% amino acid sequence homology between the deduced sequences of the Tn10 and RPl/Tnl721 tetR proteins. There is, in addition, significant amino acid sequence homology between an NH2-terminal region of the Tnl0 tetR repressor and the DNA recognition regions of other DNA-binding proteins.
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