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Nucleic Acids Research, 1984, Vol. 12, No. 12 4921-4938
© 1984

MOLECULAR BIOLOGY

Characterization of highly and moderately repetitive 500 bp Eco RI fragments from Xenopus laevis DNA

Sabine Hummel, Wolfgang Meyerhof, Elke Korge and Walter Knochel

Institut für Molekularbiologie und Biochemie, Freie Universität Berlin Arnimallee 22, D-1000 Berlin 33, FRG

Received March 28, 1984. Revised May 25, 1984. Accepted May 25, 1984.

Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain direct repeats, inverted repeats and palindromic elements. Genomic organization of the most abundant sequence (REM 1; 0.4% of total DNA) is that of an interspersed sequence. REM 2 (0.08%) is partly organized as an interspersed element and partly found in tandem arrangement, whereas REM 3 (0.02%) represents the tandemly repeated monomeric unit of a satellite DNA. In situ hybridization has shown that REM 1 and REM 2 sequences are found on most chromosomes, REM 1 being preferentially located on specific chromosomal loci. REM 3 is located near the centromere region of only one chromosome pair (presumably number 1). Hybridization of Northern blots from RNAs of different developmental stages revealed that REM 1, REM 2 and REM 3 sequences are transcribed and that transcription is under developmental control.


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