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Nucleic Acids Research, 1984, Vol. 12, No. 12 4997-5003
© 1984

MOLECULAR BIOLOGY

Structure and function of tryptophan tRNA from wheat germ

Kakoli Ghosh and Hara P. Ghosh

Department of Biochemistry, McMaster University, Hamilton Ontario L8N 3Z5, Canada

Received February 17, 1984. Revised May 16, 1984. Accepted May 16, 1984.

The coding properties of tRNATrp from yeast and wheat germ were studied. Unlike E. coli tRNATrP or mitochondrial tRNATrP, eukaryotlc tRNATrP did not recognize the UGA codon in vitro. The sequence of wheat germ tRNATrp as determined by [32p] post-labelling techniques is:

pGGAU*CCGU m1Gm2GCGCAADDGmGDAGCGCm22G{psi}CUGACmUCmCAm1GA{psi}mCAGAAGGDU GCGUGT{psi}CG m1A(A) UUCACG{psi}CGGGUCCACCAOH

The interesting features are: (i) Presence of a C11:G24 base pair in contrast to the U11:G24 in E. coli Su– tRNATrP. (ii) The anticodon sequence is -CmCA- compared to -CCA- in in E.coll tRNATrP. (iii) Lack of a hyper-modified base i6A adjacent to the 3'-end of the anticodon. (iv) Presence of -T{psi}CG- sequence instead of -{psi}{psi}CG- sequence present in mammalian tRNATrP.


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