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Nucleic Acids Research, 1984, Vol. 12, No. 13 5211-5224
© 1984

MOLECULAR BIOLOGY

The internal regulated promoter of the deo operon of Eseherichia co K-12

Poul Valentin-Hansen*, Karin Hammer1,+, Jens Erik Lø Larsen and Ib Svendsen2

Department of Molecular Biology, Odense University Campusvej 55, DK-5230 Odense M 1Institute of Biological Chemistry B, University of Copenhagen DK-2500 Valby, Denmark 2Carlsberg Laboratory, Department of Chemistry DK-2500 Valby, Denmark

*To whom reprint should be sent

Received May 14, 1984. Accepted June 20, 1984.

Previous studies of the structure and regulation of the deo operon in Escherichia coli have localized an internal regulated promoter, called deoP3, in front of the two distal genes in the operon. We report here the nucleotide sequence of the distal portion of the deoA, the deoA-deoB intercistronic region and the first part of the deoB gene, and show that deoP3 overlaps the distal segment of the deoA gene. The location of the internal promoter and the transcriptional start site were determined by means of 1) sequence homology to the concensus promoter sequence of E. coli, 2) high resolution S1 nuclease mapping of in vivo transcripts and 3) in vivo regulation of ß-galactosidase from low as well as high copy number P3lacZ protein fusion vectors.

+Formerly K.Hammer-Jespersen


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