Nucleic Acids Research, 1984, Vol. 12, No. 13 5385-5404
© 1984
MOLECULAR BIOLOGY |
The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data
Institute of Molecular Biology, USSR Academy of Sciences Moscow V-334 *Department of Molecular Genetics, Institute of Chemical Physics and Biophysics Estonian SSR Academy of Sciences, Tartu, USSR
+To whom correspondence should be addressed
Received May 10, 1984. Accepted June 12, 1984.
We have studied the accessibility of 5'–32P labeled oocyte and somatic 5S rRNAs from the fish Misgurnus fossilis L. to S1 T1 and cobra venom nucleases and have found that the cleavage sites of 5S rRNAs closely related in primary structures differ in these molecules. The data of nuclease hydrolyses revealed the existence of two conformers corresponding to renatured and partially denatured somatic 5S rRNA and capable of mutual interconversions. The exposed cytosine residues were located in oocyte and somatic 5S rRNAs converted into uridine ones by sodium bisulfite treatment. The data have been used to construct the secondary structure models of somatic and oocyte 5S rRNAs by means of specially devised computer program. These models differ in their 5'-halves which contain all the nucleotide substitutions in the primary structure, all differences in location of the exposed cytosine residues, and finally, in the cleavage pattern by the nucleases used.