Skip Navigation

This Article
Right arrow Print PDF (983K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lankhorst, P. P.
Right arrow Articles by Altona, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lankhorst, P. P.
Right arrow Articles by Altona, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 13 5419-5428
© 1984

CHEMISTRY

Carbon-13 NMR in conformational analysis of nucleic acid fragments 3. The magnitude of torsional angle in d(TpA) from CCOP and HCOP NMR coupling constants

Peter P. Lankhorst, Cornelis A.G. Haasnoot*, Cornelis Erkelens and Cornelis Altona

Gorlaeus Laboratories, State University of Leiden PO Box 9502, 2300 RA Leided *Department of Biophysical Chemistry, Toernooiveld Faculty of Science, University of Nimegen 6525 ED Nijmegen, The Netherlands

Received April 27, 1984. Accepted June 1, 1984.

Carbon-13 NMR spectra of the deoxyribonucleotide d(TpA), 3', 5'-cyclic AMP and 3', 5'-cyclic dAMP were measured. It is shown that the different substitution of C2' in deoxyribonucleotides versus ribonucleotides does not affect the vicinal C2'-C3'-03'-P coupling to a measurable extent. Therefore, the same set of Karplus parameters may be used for the C2'-C3'-03'-P couplings in ribonucleotides and in deoxyribonucleotides.

Vicinal carbon-phosphorus and proton-phosphorus coupling constants are used to calculate the magnitude of the torsion angle {varepsilon}(C4'-C3'-03'-P), which amounts to 195° in the trans conformer and to 261° in the gauche(-) conformer.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.