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Nucleic Acids Research, 1984, Vol. 12, No. 14 5609-5626
© 1984

Articles

Cloning and expression analysis of full length mouse cDNA sequences encoding the transformation associated protein p53

John R. Jenkins, Karran Rudge, Shelagh Redmond* and Alison Wade-Evans

Made Curie Memorial Foundation Research Institute The Chart, Oxted, Surrey RH8 OTL, UK

Received May 17, 1984. Accepted July 3, 1984.

We have cloned and sequenced overlapping cDNA fragment which together encode the entire mouse protein p53. Using these cDNA's we have reconstructed the full length coding region for the protein, and have analysed its coding potential by expression in vitro both as a full length sequence and as a subfragment contained in a fusion protein. The predicted amino acid sequence contains no obvious homologies to any known oncogenes but includes a possible tyrosine kinase acceptor site.

*Visiting worker


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