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Nucleic Acids Research, 1984, Vol. 12, No. 14 5627-5638
© 1984

Articles

The use of heparin as a simple cost-effective means of controlling background In nudeic acid hybridization procedures

L. Singh and K.W. Jones

Institute of Animal Genetics, University of Edinburgh Edinburgh EH9 3JN, UK

Received May 16, 1984. Revised July 3, 1984. Accepted July 3, 1984.

The use of heparin as a simple, effective and cheap substitute for conventional methods of controlling background in hybridization procedures is described and illustrated with reference to the use of DNA probes in filter and in situ hybridization. Possible specific mechanisms for this heperin effect are discussed.


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