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Nucleic Acids Research, 1984, Vol. 12, No. 14 5767-5778
© 1984

Articles

Immunological end spectroscopic studies of poly(dG-dC).poly(dG-dC) modified by cis-diamminedicholoroplatinum(II)

Jean-Marc Malinge, Marius Ptak and Marc Leng

Centre de Biophysique Moléculaire, CNRS, 1A, avenue de la Recherche Scientifique, 45045 Orleans Cedex, France

Received June 14, 1984. Accepted June 29, 1984.

The conformational changes induced by the binding of cis-diamminedichlo-roplatinum(II) to poly(dG-dC).poly(dG-dc) have been studied by reaction with specific antibodies, by circular dichroism and 3 nuclear magnetic resonan ce. Polyclonal and monoclonal antibodies to Z-DNA bind to platinated poly(dG-dC).poly(dG-dc) at low and high ionic strength. Antibodies elicited in rabbits immunized with the platinated polynucleotide bind to double stranded polynucleotides known to adopt the Z-conformation. At low and high ionic strength the circular dichroism spectrum of platinsted poly(dG-dC).poly(dG-dC) does not resemble that of poly(dG-dC) .poly(dG-dC) (B or Z conformation). At low ionic strength, the characteristic 31P nuclear magnetic resonance spectrum of the Z-form is not detected. It appears only at high ionic strength, as a component of a more complex spectrum.


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