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Nucleic Acids Research, 1984, Vol. 12, No. 14 5913-5925
© 1984

Articles

The Raman spectra of left-handed DNA oligomers incorporating adenine-thymine base pairs+

J.M. Benevides1, A.H.-J. Wang2, G.A. van der Marel3, J.H. van Boom3, A. Rich2 and G.J. Thomas, Jr.2

1Department of Chemistry, Southeastern Massachusetts University North Dartmouth, MA 02747 2Department of Biology, Massachusetts Institute of Technology Cambridge, MA 02139, USA 3Gorlaeus Laboratories, University of Leiden 2300 RA Leiden, The Netherlands

Received April 13, 1984. Revised June 18, 1984. Accepted June 18, 1984.

Reman spectra were obtained from single crystals of [d(CGCATGCG)]2 and [d(m5CGTAm5CG)]2, both of which incorporate A-T pairs into Z-DNA structures and contain C2'-endo/syn conformers of deoxyguanosine at the oligonucleotide ends. Correlation with x-ray results permits the following Reman assignments for nucleoside conformers: C3'-endo/syn C, 623±1; C2'-endolsyn C, 671±2; C end C, 782±1; C2'endo/anti T, 650±5 and a. 750; C3'-endolsyn A, 729±1 cm . These results show that (i) the 670 cm line of syn G is highly 1 sensitive to the change from C3-endo to Cr'-endo pucker, (ii) the 729 cm–1 line of A is affected neither by furanose pucker nor glycosidic bond orienta tion and (iii) the 1200-1500 cm–1 region of the Reman spectrum of the A-T double helix is greatly altered by the B-to-Z transition. Conformation sensitive Raman frequencies in the 850–1700 cm–1 region are identified for both octamer and hexamer, and the Z-to-B transition of each is monitored by spectral changes which occur upon dissolving the crystal in H2O solution.

+This is part XXVI in the series Raman Spectral Studies of Nucleic Acids. Paper XXV in this series is Benevides,J.M. and Thomas,G.J.,Jr. (1983) Nucleic Acids Rer., 11, 5747-5761.


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