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Nucleic Acids Research, 1984, Vol. 12, No. 15 6117-6125
© 1984

MOLECULAR BIOLOGY

Location of the 11 bp exon in the chicken pro {alpha}2(I) collagen gene

Sirpa Aho, Valerie Tate and Helga Boedtker

Department of Biochemistry aned Molecular Biology, Harvard University 7 Divinity Avenue, Cambridge, MA 02138, USA

Received April 17, 1984. Revised July 3, 1984. Accepted July 3, 1984.

During the fine structural analysis of the 5' end of the 38 kb chicken pro {alpha}2(I) collagen gene, we failed to locate an exon, only 11 bp in size, which had been predicted from the DNA sequence analysis of a cDNA clone complementary to the 5' end of the pro {alpha}2(I) collagen mRNA (1). We now report the location of this 11 bp exon, exon 2, at the 5' end of a 180 bp Pat I fragmant, 1900 bp 3' to exon 1 and 600 bp 5' to exon 3. Its sequence, ATGTGAGTGAG, is highly unusual in that it contains two overlapping consensus donor splice sequences. Moreover, it is flanked by two overlapping donor splice sequences but only one of the four splice sequences is actually spliced (1). The first half of intron 1 also has an unusual sequence: it is 68% GC, contains 88 CpG dinucleotides and 11 Hpa II sites. The second half is more like other intron sequences in the collagen gene with a GC content of 41%, 19 CpG, and no Hpa II sites. However it contains two sequences with 7 and 9 bp homology to the 14 bp SV40 enhancer core sequence. It is suggested that some part of intron 1 may be involved in regulation.


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