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Nucleic Acids Research, 1984, Vol. 12, No. 15 6357
© 1984

Corrigendum

Corrigendum

The unambigous determination of the |32p| labeled minor nucleotides pm1G or pt6A in tRNA is impossible by most chromatographic and electrophoretic methods, even when cold reference nucleotides are run together with the labeled ones. High voltage electrophoresis on DEAE cellulose paper run at pH 3,5 (M. Silberklang, A.M. Gillum and U.L. RajBhandary in: Methods in Enzymology (Grossman L. and Moldave K., Ed) Vol 59, pp. 58–109, Academic Press, New York and London) however permits to distinguish unambigously between these minor nucleotides.

We reinvestigated the minor nucleotide next to the anticodon of bovine tRNAsArg (anticodons C-C-U and mcm5s2U-C-{psi}) using the above mentioned electrophoretic method. We found that the nucleotide adjacent to the 3' of the anticodon has electrophoretic properties identical to those of pt6A, rather than pm1G as we published previously (G. Keith 1984 Nucleic Acids Research, 12, 2543–2547). The tRNAsArg (anticodons C-C-U and mcm5s2U-C-{psi}) have therefore t6A next to the anticodon like all other tRNAs recognising codons starting with A.

Furthermore in the title one should read mcm5s2U.


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