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Nucleic Acids Research, 1984, Vol. 12, No. 16 6359-6367
© 1984

ENZYMOLOGY

Enzymatic excision from {gamma}-irradiated polydeoxyribonucleotids of adenine residues whose imidazole rings have been rypture

Lars H. Breimer

Imperial Cancer Research Fund, Mill Hill Laboratories London NW7 1 AD, UK

Received June 29, 1984. Accepted July 24, 1984.

The main forms of base damage in polydeoxyadenylic acid {gamma}-irradiated under hypoxic conditions are due to saturation and fragmentation of the adenine imidazole ring. An irradiated polymer was annealed with an equimolar amount of poly (dT) to generate a double-stranded polydeoxyribo nucleotide containing scattered damaged base residues. On incubation of the latter with partially purified cell extracts of E.coli , imidazole ring-opened adenine, i.e. 4,6-diamino-5-formamdopyrimidine, was released in free form by a DNA glycosylase activity. The enzyme has been purified 4,500-fold, has Mr = 29,000, and appears to be identical with the previously described DNA repair enzyme formamido-pyrimidine-DNA glycosylase.


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