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Nucleic Acids Research, 1984, Vol. 12, No. 16 6523-6535
© 1984

MOLECULAR BIOLOGY

The sequence of the human immunoglobulin µ-{delta} intron reveals possible vestigial switch segments

Celia P. Milstein*, E.V. Deverson* and T.H. Rabbitts+

*AFRC Institute of Animal Physiology Babraham, Cambridge CB2 4AT +Laboratory of Molecular Biology, MRC Centre Hills Road, Cambridge CB2 2QH, UK

Received June 18, 1984. Accepted July 18, 1984.

We present the full sequence of an insert of a lambda phage clone which contains a segment of human DNA stretching from the secreted µ(µs) constant region gene through to the beginning of the constant region gene and including the membrane µ(µm) segments. The segment of 8.6kb extending from µs to the first constant domain of {delta}(C {delta}1) has been completely sequenced and reveals little conservation in comparison to the corresponding mouse sequence. The outstanding feature of the µs–µm intron is the occurrence of a potential Z-DNA forming region situated at 285bp downstream of the µs poly A addition signal. A similar DNA stretch exists in mouse and may represent a site for transcriptional control of µ gene expression. The µm–C{delta}1 intron is much longer (6Kb) than the corresponding mouse intron and includes a series of different repeats, which start at 430bp downstream of the µm poly A addition site and continue for 3.5Kb, ending about 1.5Kb from the beginning of C{delta}1. This series of repeats may be a vestigial switch sequence used in the production of the secreting cells which are the progenitors of the rare human IgD myelomas.


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