Nucleic Acids Research, 1984, Vol. 12, No. 17 6647-6661
© 1984
MOLECULAR BIOLOGY |
The isolation of a human Ig V
gene from a recombinant library of chromosome 22 and estimation of its copy number
Beatson Institute, Garscube Estate Switchback Road, Bearsden, Glasgow, UK +Institut de Pathologie Moléculaire, INSERM U 129, Faculté de Médecine, Cochin Port-Royal, 75014 Paris
Received August 6, 1984. Accepted August 21, 1984.
We report the first isolation and characterisation of a human Ig V
gene. The gene was isolated from a recombinant phage library of human chromosome 22 using a mouse cDNA as probe. DNA sequence analysis predicts a short leader peptide interrupted by an intron of 88 nucleotides, and a mature polypeptide of 96–97 amino acids which shares 61% homology with mouse VI chains. Comparison with the amino acids sequence of known human chains of all six subgroups shows agreement at 22/25 low variance positions. However the maximum homology with human chains is 49%, so we conclude that this sequence represents a new IgV subgroup. The coding region is followed by the conserved heptamer, CACAGTG, and nonamer, ACATAAACC, sequences which have been implicated in V-J segment recombination. This gene has the hallmarks of an active V gene including recently identified transcriptional controlling sequences. Probing genomic DNA with the subcloned V gene detects a family of about 10 cross hybridising members at low stringency and 2 at high stringency. There is limited polymorphism of the V locus.
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